Characterization of enterotoxigenic Escherichia coli heat-labile toxin (LT) double mutant LTA72R/R192G as a Nontoxic and Effective Mucosal Adjuvant

Li Ben-Qiang, Tao Jie, Cheng Jing-Hua, Shi Ying and Liu Hui-li*

The heat-labile enterotoxins of Escherichia coli (LT) protein were reported to be an ideal mucosal adjuvant for nasal or oral delivery with antigen. Because of its toxicity, the application of native LT protein was focused on the purification of subunit B (LTB) or reconstructing non-toxic LT mutants, such as LTK63, LTR72, LTG192, or LTK63/R72. In this study, we mutated nucleotides coding the rd63rd and nd72nd residues, as well as the nd72nd, and 192th amino acids, to explore whether the double mutant LTA72R/R192G had a good adjuvanticity the same as the single mutant LTS63K or LTA72R, which were known as the ideal mucosal adjuvants. LTA72R/R192G was harmless to the mice because of the reduction of the ADP- ribosylation activity and toxicity. Besides, it could significantly enhance the mucosal immune response against the NCDV or CSFV antigens. In conclusion, the nontoxicity LTA72R/R192G may potentially serve as an effective adjuvant for mucosal immunization. 

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Published on: Oct 26, 2022 Pages: 33-39

Full Text PDF Full Text HTML DOI: 10.17352/jvi.000053
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