Study of Immunogenicity and Protective Efficacy of Live MDCK-derived Pandemic Influenza Vaccine

Elena A Nechaeva*, Ryzhikov AB, Pyankova OG, Radaeva IF, Pyankov OV, Danilchenko NV, Agafonov AP, Kiseleva LV, Larionova NV and Rudenko LG

Background: The threat of pandemic A/H1N1 influenza is still a matter of considerable public concern. Influenza outbreak in 2009 underlined the importance of rapid production of a sufficient vaccine reserve for pandemic and interpandemic periods. One promising way to allay this concern is development of cell culture-derived live attenuated influenza vaccines (LAIV), because this technology makes it possible to produce a considerable amount of vaccine over a short period of time.

Objectives: The goal of this work was to study the immunogenic and protective properties of the Vector-Flu vaccine in animal models.

Methods: We have developed Vector-Flu, a live attenuated vaccine against pandemic influenza, based on the cold-adapted reassortant vaccine strain for LAIV, A/17/California/2009/38(H1N1), and produced in certified MDCK cells. The immunogenicity and protective efficacy of Vector-Flu vaccine were studied in ferrets and mice.

Results: A double immunization with the live MDCK-derived Vector-Flu influenza vaccine induced a high level of neutralizing antibodies in ferret serum both to the pandemic A/Chita/3/2009(H1N1) influenza virus strain, isolated in Russia, and to the pandemic A/California/7/2009(H1N1) strain. Intranasal immunization of mice induced levels of serum antibodies sufficient to protect them when aerosol-challenged with 100 infectious doses of A/Chita/3/2009(H1N1) strain. 

Conclusion: Theoretical estimates of protective antibody levels necessary for protecting humans from the disease caused by pandemic A/H1N1(2009) influenza virus, and the experimental data from animal models (ferrets and mice), suggest that the Vector-Flu vaccine is able to protect humans after a single immunization.

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Published on: Jun 6, 2019 Pages: 10-15

Full Text PDF Full Text HTML DOI: 10.17352/2455-5363.000023
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